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third generation lentivirus packaging plasmids  (Addgene inc)


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    Addgene inc third generation lentivirus packaging plasmids
    Third Generation Lentivirus Packaging Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2735 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/third generation lentivirus packaging plasmids/product/Addgene inc
    Average 96 stars, based on 2735 article reviews
    third generation lentivirus packaging plasmids - by Bioz Stars, 2026-02
    96/100 stars

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    Inhibition of the cell entry of SARS-CoV-2 pseudotyped virus particles. 293T cells transiently expressing human ACE2 and human TMPRSS2a, were pre-treated with the selected compounds at the concentrations indicated for 3 h or with 20 μg / ml of the human anti-ACE2 antibody (positive control), before they were inoculated with SARS-CoV-2-specific spike protein pseudotyped lentivirus particles (spike virus) or particles without a viral envelope (Δ spike). At 48 h postinoculation, pseudotyped virus entry was analyzed by luminescence readout (normalization against untreated spike virus entry). Data represent the mean±SD from three independent experiments carried out in technical triplicates. One biological experiment of otamixaban was excluded due to technical error.

    Journal: bioRxiv

    Article Title: Entrectinib - a SARS-CoV-2 inhibitor in Human Lung Tissue (HLT) cells

    doi: 10.1101/2021.09.07.459123

    Figure Lengend Snippet: Inhibition of the cell entry of SARS-CoV-2 pseudotyped virus particles. 293T cells transiently expressing human ACE2 and human TMPRSS2a, were pre-treated with the selected compounds at the concentrations indicated for 3 h or with 20 μg / ml of the human anti-ACE2 antibody (positive control), before they were inoculated with SARS-CoV-2-specific spike protein pseudotyped lentivirus particles (spike virus) or particles without a viral envelope (Δ spike). At 48 h postinoculation, pseudotyped virus entry was analyzed by luminescence readout (normalization against untreated spike virus entry). Data represent the mean±SD from three independent experiments carried out in technical triplicates. One biological experiment of otamixaban was excluded due to technical error.

    Article Snippet: Third generation lentivirus packaging plasmids - pLenti CMV Puro Luc (17477), pMDLg.pRRE (12251), pRSV.REV (12253), pMD2.G (12259) were purchased from Addgene.

    Techniques: Inhibition, Virus, Expressing, Positive Control

    Inhibition of the cell entry of vesicular stomatitis virus G (VSV.G) pseudotyped particles. 293T cells transiently expressing human ACE2 and human TMPRSS2a, were pre-treated with the selected compounds at 10 μM for 3 h before they were inoculated with VSV.G envelope protein pseudotyped lentivirus particles. At 48 h postinoculation, pseudotyped virus entry was analyzed by luminescence readout (normalization against untreated VSV.G virus entry). Data represent the mean±SD from three independent experiments carried out in technical triplicates.

    Journal: bioRxiv

    Article Title: Entrectinib - a SARS-CoV-2 inhibitor in Human Lung Tissue (HLT) cells

    doi: 10.1101/2021.09.07.459123

    Figure Lengend Snippet: Inhibition of the cell entry of vesicular stomatitis virus G (VSV.G) pseudotyped particles. 293T cells transiently expressing human ACE2 and human TMPRSS2a, were pre-treated with the selected compounds at 10 μM for 3 h before they were inoculated with VSV.G envelope protein pseudotyped lentivirus particles. At 48 h postinoculation, pseudotyped virus entry was analyzed by luminescence readout (normalization against untreated VSV.G virus entry). Data represent the mean±SD from three independent experiments carried out in technical triplicates.

    Article Snippet: Third generation lentivirus packaging plasmids - pLenti CMV Puro Luc (17477), pMDLg.pRRE (12251), pRSV.REV (12253), pMD2.G (12259) were purchased from Addgene.

    Techniques: Inhibition, Virus, Expressing